Equivalence class NR_20.0_30925.2 Obsolete
| # | IFE | Standardized name | Molecule | Organism | Source | Rfam | Title | Method | Å | Date |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 4ZT0|1|D (rep) | single-guide RNA | Streptococcus pyogenes | Bacteria | Crystal structure of catalytically-active Streptococcus pyogenes Cas9 in complex with single-guide RNA at 2.9 Angstrom resolution | X-ray diffraction | 2.9 | 2015-07-08 | ||
| 2 | 4ZT0|1|B | single-guide RNA | Streptococcus pyogenes | Bacteria | Crystal structure of catalytically-active Streptococcus pyogenes Cas9 in complex with single-guide RNA at 2.9 Angstrom resolution | X-ray diffraction | 2.9 | 2015-07-08 | ||
| 3 | 4ZT9|1|D | single-guide RNA | Streptococcus pyogenes | Bacteria | Nuclease-inactive Streptococcus pyogenes Cas9 (D10A/H840A, dCas9) in complex with single-guide RNA at 3.1 Angstrom resolution | X-ray diffraction | 3.1 | 2015-07-08 | ||
| 4 | 4ZT9|1|B | single-guide RNA | Streptococcus pyogenes | Bacteria | Nuclease-inactive Streptococcus pyogenes Cas9 (D10A/H840A, dCas9) in complex with single-guide RNA at 3.1 Angstrom resolution | X-ray diffraction | 3.1 | 2015-07-08 | ||
| 5 | 7S4X|1|B | gRNA | synthetic construct | Synthetic | Cas9:gRNA in complex with 18-20MM DNA, 1 minute time-point, kinked active conformation | Electron microscopy | 2.76 | 2022-03-02 | ||
| 6 | 6O0X|1|B | single guide RNA | synthetic construct | Synthetic | Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium | Electron microscopy | 3.28 | 2019-07-10 | ||
| 7 | 7S4V|1|B | gRNA | synthetic construct | Synthetic | Cas9 bound to 12-14MM DNA, 60 min time-point, kinked conformation | Electron microscopy | 3.28 | 2022-03-02 | ||
| 8 | 6O0Y|1|B | single guide RNA | synthetic construct | Synthetic | Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium | Electron microscopy | 3.37 | 2019-07-10 | ||
| 9 | 6O0Z|1|B | single guide RNA | synthetic construct | Synthetic | Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium | Electron microscopy | 3.3 | 2019-07-10 | ||
| 10 | 7S4U|1|B | gRNA | synthetic construct | Synthetic | Cryo-EM structure of Cas9 in complex with 12-14MM DNA substrate, 5 minute time-point | Electron microscopy | 3.56 | 2022-03-02 |
Release history
| Release | 3.220 | 3.221 | 3.222 | 3.223 | 3.224 | 3.225 | 3.226 | 3.227 | 3.228 | 3.229 | 3.230 | 3.231 | 3.232 | 3.233 | 3.234 | 3.235 | 3.236 | 3.237 | 3.238 | 3.239 | 3.240 | 3.241 | 3.242 | 3.243 | 3.244 | 3.245 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Date | 2022-03-02 | 2022-03-09 | 2022-03-16 | 2022-03-23 | 2022-03-30 | 2022-04-06 | 2022-04-13 | 2022-04-20 | 2022-04-27 | 2022-05-04 | 2022-05-11 | 2022-05-18 | 2022-05-25 | 2022-06-01 | 2022-06-08 | 2022-06-15 | 2022-06-22 | 2022-06-29 | 2022-07-06 | 2022-07-13 | 2022-07-20 | 2022-07-27 | 2022-08-03 | 2022-08-10 | 2022-08-17 | 2022-08-24 |
Parents
Children
| This class | Descendant classes | Release id | Intersection | Only in this class | Added to child |
|---|---|---|---|---|---|
| NR_20.0_30925.2 | NR_20.0_20965.1 | 3.246 | (10) 7S4V|1|B, 4ZT0|1|B, 4ZT9|1|B, 4ZT9|1|D, 6O0Y|1|B, 7S4U|1|B, 6O0Z|1|B, 7S4X|1|B, 4ZT0|1|D, 6O0X|1|B | (0) | (10) 7Z4J|1|A, 7Z4E|1|A, 7Z4L|1|A, 7Z4H|1|A, 7Z4D|1|A, 7Z4I|1|A, 7Z4D|1|F, 7Z4K|1|A, 7Z4G|1|A, 7Z4C|1|A |
Heat map of mutual geometric discrepancy, in Angstroms per nucleotide. Instances are ordered to put similar structures near each other. The colorbar ranges from 0 to the maximum observed discrepancy, up to 0.5
#S - ordering by similarity (same as in the heat map).| #S | PDB | Title | Method | Resolution | Length |
|---|---|---|---|---|---|
| 1 | 4ZT9|1|D | Nuclease-inactive Streptococcus pyogenes Cas9 (D10A/H840A, dCas9) in complex with single-guide RNA at 3.1 Angstrom resolution | X-RAY DIFFRACTION | 3.1 | 72 |
| 2 | 4ZT0|1|D | Crystal structure of catalytically-active Streptococcus pyogenes Cas9 in complex with single-guide RNA at 2.9 Angstrom resolution | X-RAY DIFFRACTION | 2.9 | 72 |
| 3 | 4ZT0|1|B | Crystal structure of catalytically-active Streptococcus pyogenes Cas9 in complex with single-guide RNA at 2.9 Angstrom resolution | X-RAY DIFFRACTION | 2.9 | 72 |
| 4 | 4ZT9|1|B | Nuclease-inactive Streptococcus pyogenes Cas9 (D10A/H840A, dCas9) in complex with single-guide RNA at 3.1 Angstrom resolution | X-RAY DIFFRACTION | 3.1 | 72 |
| 5 | 6O0Y|1|B | Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium | ELECTRON MICROSCOPY | 3.37 | 98 |
| 6 | 6O0X|1|B | Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium | ELECTRON MICROSCOPY | 3.28 | 98 |
| 7 | 7S4V|1|B | Cas9 bound to 12-14MM DNA, 60 min time-point, kinked conformation | ELECTRON MICROSCOPY | 3.28 | 98 |
| 8 | 7S4X|1|B | Cas9:gRNA in complex with 18-20MM DNA, 1 minute time-point, kinked active conformation | ELECTRON MICROSCOPY | 2.76 | 98 |
| 9 | 6O0Z|1|B | Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium | ELECTRON MICROSCOPY | 3.3 | 96 |
| 10 | 7S4U|1|B | Cryo-EM structure of Cas9 in complex with 12-14MM DNA substrate, 5 minute time-point | ELECTRON MICROSCOPY | 3.56 | 97 |