HL_5B2S_003

3D structure

PDB id

5B2S (explore in PDB, NAKB, or RNA 3D Hub)

Description

Crystal structure of the Streptococcus pyogenes Cas9 EQR variant in complex with sgRNA and target DNA (TGAG PAM)

Experimental method

X-RAY DIFFRACTION

Resolution

2.2 Å

Loop

Sequence

UGAAAA

Length

6 nucleotides

Bulged bases

None detected

QA status

Unknown status

Sequence variability

If this chain is mapped to an Rfam alignment, the link below will give its sequence variability.

R3DSVS

Structural variability across Equivalence Class

The link below will give the loop's structural variability across the equivalence class for this chain.

R3DMCS EC

Structural variability across Rfam

If this chain is mapped to an Rfam alignment, the link below will give the loop's structural variability between chains mapped to the same Rfam family.

R3DMCS Rfam

HL_5B2S_003 not in the Motif Atlas

Geometric match to HL_3E5C_001

Geometric discrepancy: 0.1575

The information below is about HL_3E5C_001

Detailed Annotation

GNRA

Broad Annotation

No text annotation

Motif group

HL_34789.2

Basepair signature

cWW-F-F-F-F

Number of instances in this motif group

397

Unit IDs

5B2S|1|A|U|72
5B2S|1|A|G|73
5B2S|1|A|A|74
5B2S|1|A|A|75
5B2S|1|A|A|76
5B2S|1|A|A|77

Current chains

Chain A

Guide RNA

Nearby chains

Chain B

CRISPR-associated endonuclease Cas9