IL_5B2P_003

3D structure

PDB id

5B2P (explore in PDB, NAKB, or RNA 3D Hub)

Description

Crystal structure of Francisella novicida Cas9 in complex with sgRNA and target DNA (TGA PAM)

Experimental method

X-RAY DIFFRACTION

Resolution

1.7 Å

Loop

Sequence

CCUCU*ACG

Length

8 nucleotides

Bulged bases

5B2P|1|B|C|89

QA status

Valid loop

Sequence variability

If this chain is mapped to an Rfam alignment, the link below will give its sequence variability.

R3DSVS

Structural variability across Equivalence Class

The link below will give the loop's structural variability across the equivalence class for this chain.

R3DMCS EC

Structural variability across Rfam

If this chain is mapped to an Rfam alignment, the link below will give the loop's structural variability between chains mapped to the same Rfam family.

R3DMCS Rfam

Detailed Annotation

C-loop

Broad Annotation

No text annotation

Motif group

IL_63596.9

Basepair signature

cWW-cWS-cHS-L-cWW-tWH

Number of instances in this motif group

15

Unit IDs

5B2P|1|B|C|76
5B2P|1|B|C|77
5B2P|1|B|U|78
5B2P|1|B|C|79
5B2P|1|B|U|80
*
5B2P|1|B|A|88
5B2P|1|B|C|89
5B2P|1|B|G|90

Current chains

Chain B

Guide RNA

Nearby chains

Chain A

CRISPR-associated endonuclease Cas9

Chain C

Target DNA