IL_5B2T_002

3D structure

PDB id

5B2T (explore in PDB, NAKB, or RNA 3D Hub)

Description

Crystal structure of the Streptococcus pyogenes Cas9 VRER variant in complex with sgRNA and target DNA (TGCG PAM)

Experimental method

X-RAY DIFFRACTION

Resolution

2.2 Å

Loop

Sequence

GC*GUC

Length

5 nucleotides

Bulged bases

5B2T|1|A|U|59

QA status

Valid loop

Sequence variability

If this chain is mapped to an Rfam alignment, the link below will give its sequence variability.

R3DSVS

Structural variability across Equivalence Class

The link below will give the loop's structural variability across the equivalence class for this chain.

R3DMCS EC

Structural variability across Rfam

If this chain is mapped to an Rfam alignment, the link below will give the loop's structural variability between chains mapped to the same Rfam family.

R3DMCS Rfam

Detailed Annotation

Single bulged U

Broad Annotation

No text annotation

Motif group

IL_83039.19

Basepair signature

cWW-L-cWW

Number of instances in this motif group

123

Unit IDs

5B2T|1|A|G|54
5B2T|1|A|C|55
*
5B2T|1|A|G|58
5B2T|1|A|U|59
5B2T|1|A|C|60

Current chains

Chain A

Guide RNA

Nearby chains

Chain B

CRISPR-associated endonuclease Cas9