Equivalence class NR_4.0_05245.1 Current
| # | IFE | Standardized name | Molecule | Organism | Source | Rfam | Title | Method | Res. Å | #NTs | Date |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 8SFO|1|B+ 8SFO|1|C+ 8SFO|1|D (rep) | DNA (35-MER), DNA (40-MER), RNA (39-MER) | synthetic construct | WT CRISPR-Cas12a with a 20bp R-loop and nontarget strand in the RuvC active site. | Electron microscopy | 3.3 | 119 | 2024-07-03 | |||
| 2 | 8SFL|1|B+ 8SFL|1|C+ 8SFL|1|D | DNA (40-MER), RNA (34-MER) | synthetic construct | WT CRISPR-Cas12a with a 15bp R-loop | Electron microscopy | 3.3 | 114 | 2024-07-03 | |||
| 3 | 8SFN|1|B+ 8SFN|1|C+ 8SFN|1|D | DNA (30-MER), DNA (31-MER), RNA (36-MER) | synthetic construct | WT CRISPR-Cas12a with a 16bp R-loop and nontarget strand in the RuvC active site. | Electron microscopy | 3.3 | 104 | 2024-07-03 | |||
| 4 | 8SFJ|1|B+ 8SFJ|1|C+ 8SFJ|1|D | DNA (5'-D(P*CP*AP*CP*TP*TP*AP*TP*CP*AP*CP*TP*AP*AP*AP*AP*GP*AP*TP*CP*GP*GP*AP*AP*G)-3'), DNA (5'-D(P*CP*TP*TP*CP*CP*GP*AP*TP*CP*TP*TP*TP*TP*AP*GP*TP*GP*AP*T)-3'), RNA (29-MER) | synthetic construct | WT CRISPR-Cas12a with a 10bp R-loop | Electron microscopy | 3.6 | 97 | 2024-07-03 | |||
| 5 | 8SFQ|1|B+ 8SFQ|1|C+ 8SFQ|1|D | DNA (34-MER), DNA (5'-D(P*CP*TP*TP*CP*CP*GP*AP*TP*CP*TP*TP*TP*TP*AP*GP*TP*GP*AP*T)-3'), RNA (39-MER) | synthetic construct | WT CRISPR-Cas12a post nontarget strand-cleavage with the the RuvC active site exposed. | Electron microscopy | 3.5 | 92 | 2024-07-03 | |||
| 6 | 8SFR|1|B+ 8SFR|1|C+ 8SFR|1|D | DNA (34-MER), DNA (5'-D(P*CP*TP*TP*CP*CP*GP*AP*TP*CP*TP*TP*TP*TP*AP*GP*TP*GP*AP*T)-3'), RNA (39-MER) | synthetic construct | WT CRISPR-Cas12a post nontarget strand cleavage. | Electron microscopy | 3.5 | 92 | 2024-07-03 | |||
| 7 | 8SFP|1|B+ 8SFP|1|C+ 8SFP|1|D | DNA (40-MER), DNA (5'-D(P*CP*TP*TP*CP*CP*GP*AP*TP*CP*TP*TP*TP*TP*AP*GP*TP*GP*AP*TP*A)-3'), RNA (39-MER) | synthetic construct | WT CRISPR-Cas12a with the target strand in the RuvC active site. | Electron microscopy | 3.8 | 93 | 2024-07-03 | |||
| 8 | 8SFI|1|B+ 8SFI|1|C+ 8SFI|1|D | DNA (5'-D(P*CP*TP*TP*AP*TP*CP*AP*CP*TP*AP*AP*AP*AP*GP*AP*TP*CP*GP*GP*AP*AP*G)-3'), DNA (5'-D(P*CP*TP*TP*CP*CP*GP*AP*TP*CP*TP*TP*TP*TP*AP*GP*TP*GP*AP*TP*A)-3'), RNA (28-MER) | synthetic construct | WT CRISPR-Cas12a with a 8bp R-loop | Electron microscopy | 3.5 | 70 | 2024-07-03 |
Release history
Parents
| This class | Parent classes | Release id | Intersection | Added to this class | Only in parent |
|---|
Children
| This class | Descendant classes | Release id | Intersection | Only in this class | Added to child |
|---|
Instances are ordered to put similar structures near each other. Select one instance to see its 3D structure. Selecting two or more instances will show their superposition, but only chains with identical numbers of observed nucleotides will superpose well. Large structures are slow to display; this tool is not designed for that.
| #S | View | PDB | Title | Method | Resolution | #NTs |
|---|---|---|---|---|---|---|
| 1 | 8SFR|1|B+ 8SFR|1|C+ 8SFR|1|D | WT CRISPR-Cas12a post nontarget strand cleavage. | ELECTRON MICROSCOPY | 3.5 | 92 | |
| 2 | 8SFP|1|B+ 8SFP|1|C+ 8SFP|1|D | WT CRISPR-Cas12a with the target strand in the RuvC active site. | ELECTRON MICROSCOPY | 3.8 | 93 | |
| 3 | 8SFQ|1|B+ 8SFQ|1|C+ 8SFQ|1|D | WT CRISPR-Cas12a post nontarget strand-cleavage with the the RuvC active site exposed. | ELECTRON MICROSCOPY | 3.5 | 92 | |
| 4 | 8SFO|1|B+ 8SFO|1|C+ 8SFO|1|D | WT CRISPR-Cas12a with a 20bp R-loop and nontarget strand in the RuvC active site. | ELECTRON MICROSCOPY | 3.3 | 119 | |
| 5 | 8SFN|1|B+ 8SFN|1|C+ 8SFN|1|D | WT CRISPR-Cas12a with a 16bp R-loop and nontarget strand in the RuvC active site. | ELECTRON MICROSCOPY | 3.3 | 104 | |
| 6 | 8SFJ|1|B+ 8SFJ|1|C+ 8SFJ|1|D | WT CRISPR-Cas12a with a 10bp R-loop | ELECTRON MICROSCOPY | 3.6 | 97 | |
| 7 | 8SFI|1|B+ 8SFI|1|C+ 8SFI|1|D | WT CRISPR-Cas12a with a 8bp R-loop | ELECTRON MICROSCOPY | 3.5 | 70 | |
| 8 | 8SFL|1|B+ 8SFL|1|C+ 8SFL|1|D | WT CRISPR-Cas12a with a 15bp R-loop | ELECTRON MICROSCOPY | 3.3 | 114 |
Heat map of mutual geometric discrepancy, in Angstroms per nucleotide. The ordering in the heat map is the same as in the table. The colorbar ranges from 0 to the maximum observed discrepancy. Click above the diagonal to select a range of structures, below the diagonal to select two structures.
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