1 | 6WBR|1|B+ 6WBR|1|C+ 6WBR|1|D (rep) | | DNA (30-MER), DNA (5'-D(*AP*TP*AP*CP*CP*TP*GP*GP*CP*G)-3'), RNA (94-MER) | Acidothermus cellulolyticus 11B | | | Crystal structure of AceCas9 bound with guide RNA and DNA with 5'-NNNCC-3' PAM | X-ray diffraction | 2.91 | 131 | 2020-11-18 |
2 | 6WC0|1|B+ 6WC0|1|C+ 6WC0|1|D | | DNA (30-MER), DNA (5'-D(*AP*TP*AP*CP*TP*TP*GP*GP*CP*G)-3'), sgRNA (95-MER) | Acidothermus cellulolyticus 11B | | | Crystal structure of AceCas9 bound with guide RNA and DNA with 5'-NNNTC-3' PAM | X-ray diffraction | 3.61 | 131 | 2020-11-18 |
3 | 8D2L|1|B+ 8D2L|1|T+ 8D2L|1|D+ 8D2L|1|X | | DNA non-target strand (5'-D(P*AP*GP*A)-3'), DNA non-target strand (5'-D(P*TP*AP*TP*AP*CP*AP*CP*CP*AP*AP*GP*CP*T)-3'), DNA target strand (5'-D(*AP*GP*CP*TP*TP*GP*GP*TP*GP*TP*AP*TP*A)-3'), DNA target strand (5'-D(P*CP*CP*AP*GP*GP*AP*TP*CP*TP*TP*GP*CP*CP*AP*TP*CP*CP*TP*AP*CP*CP*TP*CP*T)-3'), Single guide RNA (106-MER) | Acidothermus cellulolyticus 11B | | | Structure of Acidothermus cellulolyticus Cas9 ternary complex (Cleavage Intermediate 1) | Electron microscopy | 2.21 | 147 | 2023-12-20 |
4 | 8D2K|1|B+ 8D2K|1|T+ 8D2K|1|D+ 8D2K|1|X | | DNA non-target strand (5'-D(P*AP*GP*A)-3'), DNA non-target strand (5'-D(P*TP*AP*TP*AP*CP*AP*CP*CP*AP*AP*GP*CP*T)-3'), DNA target strand (5'-D(*AP*GP*CP*TP*TP*GP*GP*TP*GP*TP*AP*TP*A)-3'), DNA target strand (5'-D(P*CP*CP*AP*GP*GP*AP*TP*CP*TP*TP*GP*CP*CP*AP*TP*CP*CP*TP*AP*CP*CP*TP*CP*T)-3'), Single Guide RNA (102-MER) | Acidothermus cellulolyticus | | | Structure of Acidothermus cellulolyticus Cas9 ternary complex (Cleavage Intermediate 2) | Electron microscopy | 2.43 | 152 | 2023-12-20 |
5 | 8D2O|1|B+ 8D2O|1|X+ 8D2O|1|D+ 8D2O|1|T | | DNA non-target strand (5'-D(P*AP*TP*AP*CP*AP*CP*CP*AP*AP*GP*CP*T)-3'), DNA target strand (5'-D(*AP*GP*CP*TP*TP*GP*GP*TP*GP*TP*AP*TP*A)-3'), DNA target strand (5'-D(P*CP*CP*AP*GP*GP*AP*TP*CP*TP*TP*G)-3'), Single guide RNA (106-MER) | Acidothermus cellulolyticus 11B | | | Structure of Acidothermus cellulolyticus Cas9 ternary complex (Post-cleavage 2) | Electron microscopy | 2.66 | 123 | 2023-12-20 |
6 | 8D2Q|1|B+ 8D2Q|1|X+ 8D2Q|1|D+ 8D2Q|1|T | | DNA non-target strand (5'-D(P*AP*TP*AP*CP*AP*CP*CP*AP*AP*GP*CP*T)-3'), DNA target strand (5'-D(*AP*GP*CP*TP*TP*GP*GP*TP*GP*TP*AP*TP*A)-3'), DNA target strand (5'-D(P*CP*CP*AP*GP*GP*AP*TP*CP*TP*TP*G)-3'), Single Guide RNA (86-MER) | Acidothermus cellulolyticus 11B | | | Structure of Acidothermus cellulolyticus Cas9 ternary complex (Post-cleavage 1) | Electron microscopy | 2.58 | 122 | 2023-12-20 |
7 | 8D2P|1|B+ 8D2P|1|T | | DNA target strand (5'-D(P*CP*CP*AP*GP*GP*AP*TP*CP*TP*TP*GP*CP*CP*AP*TP*CP*CP*TP*AP*CP*CP*TP*CP*T)-3'), Single guide RNA (102-MER) | Acidothermus cellulolyticus 11B | | | Structure of Acidothermus cellulolyticus Cas9 ternary complex (Target bound) | Electron microscopy | 2.78 | 126 | 2023-12-20 |
8 | 8D2N|1|B+ 8D2N|1|X+ 8D2N|1|D | | DNA non-target strand (5'-D(P*TP*AP*CP*AP*CP*CP*AP*AP*GP*CP*T)-3'), DNA target strand (25-MER), Single guide RNA (106-MER) | Acidothermus cellulolyticus 11B | | | Structure of Acidothermus cellulolyticus Cas9 ternary complex (Pre-cleavage) | Electron microscopy | 2.88 | 128 | 2023-12-20 |
9 | 9AR5|1|B+ 9AR5|1|X+ 9AR5|1|D | | DNA (25-MER), DNA/RNA (5'-D(P*TP*AP*CP*A)-R(P*(5MC))-D(P*CP*AP*AP*GP*CP*T)-3'), RNA (92-MER) | Acidothermus cellulolyticus | | | Structure of Acidothermus cellulolyticus Cas9 bound with methylated DNA | Electron microscopy | 2.88 | 128 | 2025-02-26 |