1 | 5B2T|1|A+ 5B2T|1|C+ 5B2T|1|D (rep) | Trans-activating crRNA | Guide RNA, Non-target DNA, DNA (5'-D(*TP*GP*CP*GP*AP*TP*TP*G)-3'), Target DNA | Streptococcus pyogenes | Bacteria | RF02348 | Crystal structure of the Streptococcus pyogenes Cas9 VRER variant in complex with sgRNA and target DNA (TGCG PAM) | X-ray diffraction | 2.2 | 117 | 2016-03-23 |
2 | 5B2R|1|A+ 5B2R|1|C+ 5B2R|1|D | Trans-activating crRNA | Guide RNA, Non-target DNA, DNA (5'-D(*TP*GP*AP*GP*AP*TP*TP*G)-3'), Target DNA | Streptococcus pyogenes | Bacteria | RF02348 | Crystal structure of the Streptococcus pyogenes Cas9 VQR variant in complex with sgRNA and target DNA (TGA PAM) | X-ray diffraction | 2 | 117 | 2016-03-23 |
3 | 5B2S|1|A+ 5B2S|1|C+ 5B2S|1|D | Trans-activating crRNA | Guide RNA, Non-target DNA, DNA (5'-D(*TP*GP*AP*GP*AP*TP*TP*G)-3'), Target DNA | Streptococcus pyogenes | Bacteria | RF02348 | Crystal structure of the Streptococcus pyogenes Cas9 EQR variant in complex with sgRNA and target DNA (TGAG PAM) | X-ray diffraction | 2.2 | 117 | 2016-03-23 |
4 | 6AI6|1|B+ 6AI6|1|C+ 6AI6|1|D | Trans-activating crRNA | DNA (28-MER), DNA (8-MER), RNA (81-MER) | Streptococcus pyogenes | Bacteria | RF02348 | Crystal structure of SpCas9-NG | X-ray diffraction | 2.7 | 117 | 2018-10-31 |
5 | 4OO8|1|B+ 4OO8|1|C | | DNA (5'-D(*CP*CP*AP*GP*CP*CP*AP*AP*GP*CP*GP*CP*AP*CP*CP*TP*AP*AP*TP*TP*TP*CP*C)-3'), RNA (97-MER) | | | | Crystal structure of Streptococcus pyogenes Cas9 in complex with guide RNA and target DNA | X-ray diffraction | 2.5 | 118 | 2014-02-26 |
6 | 4OO8|1|E+ 4OO8|1|F | | DNA (5'-D(*CP*CP*AP*GP*CP*CP*AP*AP*GP*CP*GP*CP*AP*CP*CP*TP*AP*AP*TP*TP*TP*CP*C)-3'), RNA (97-MER) | | | | Crystal structure of Streptococcus pyogenes Cas9 in complex with guide RNA and target DNA | X-ray diffraction | 2.5 | 120 | 2014-02-26 |
7 | 5XBL|1|B | Trans-activating crRNA | RNA (98-MER) | Streptococcus pyogenes | Bacteria | RF02348 | Structure of nuclease in complex with associated protein | X-ray diffraction | 3.05 | 88 | 2017-06-14 |
8 | 6IFO|1|C | Trans-activating crRNA | RNA (99-MER) | Streptococcus pyogenes | Bacteria | RF02348 | Crystal structure of AcrIIA2-SpyCas9-sgRNA ternary complex | X-ray diffraction | 3.31 | 88 | 2019-01-23 |
9 | 6IFO|1|D | Trans-activating crRNA | RNA (99-MER) | Streptococcus pyogenes | Bacteria | RF02348 | Crystal structure of AcrIIA2-SpyCas9-sgRNA ternary complex | X-ray diffraction | 3.31 | 90 | 2019-01-23 |
10 | 8SRS|1|B+ 8SRS|1|D+ 8SRS|1|c+ 8SRS|1|C | Trans-activating crRNA | gRNA, NTS, TS | Streptococcus pyogenes | Bacteria | RF02348 | SpRY-Cas9:gRNA complex targeting TAC PAM DNA | Electron microscopy | 2.79 | 149 | 2024-05-01 |
11 | 8T7S|1|B+ 8T7S|1|C+ 8T7S|1|D+ 8T7S|1|c | Trans-activating crRNA | gRNA, NTS, TS | Streptococcus pyogenes | Bacteria | RF02348 | SpRYmer bound to NAC PAM DNA | Electron microscopy | 3.01 | 151 | 2024-05-01 |
12 | 8SPQ|1|B+ 8SPQ|1|C+ 8SPQ|1|D+ 8SPQ|1|E | Trans-activating crRNA | gRNA, NTS, TS | Streptococcus pyogenes | Bacteria | RF02348 | SpRY-Cas9:gRNA complex targeting TGG PAM DNA | Electron microscopy | 3.26 | 149 | 2024-05-01 |
13 | 8T6X|1|B+ 8T6X|1|C+ 8T6X|1|D | Trans-activating crRNA | gRNA, NTS, TS | Streptococcus pyogenes | Bacteria | RF02348 | SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 18 bp R-loop | Electron microscopy | 3.08 | 139 | 2024-05-01 |
14 | 8T6T|1|B+ 8T6T|1|C+ 8T6T|1|D | Trans-activating crRNA | gRNA, NTS, TS | Streptococcus pyogenes | Bacteria | RF02348 | SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 13 bp R-loop | Electron microscopy | 3.04 | 129 | 2024-05-01 |
15 | 8SQH|1|B+ 8SQH|1|D+ 8SQH|1|c+ 8SQH|1|C | Trans-activating crRNA | gRNA, NTS, TS | Streptococcus pyogenes | Bacteria | RF02348 | SpRY-Cas9:gRNA complex targeting TTC PAM DNA | Electron microscopy | 3.69 | 147 | 2024-05-01 |
16 | 8T79|1|B+ 8T79|1|C+ 8T79|1|D | Trans-activating crRNA | gRNA, NTS, TS | Streptococcus pyogenes | Bacteria | RF02348 | SpRY-Cas9:gRNA complex bound to non-target DNA with 10 bp R-loop | Electron microscopy | 3.04 | 118 | 2024-05-08 |
17 | 8T78|1|B+ 8T78|1|C+ 8T78|1|D | Trans-activating crRNA | gRNA, NTS, TS | Streptococcus pyogenes | Bacteria | RF02348 | SpRY-Cas9:gRNA complex bound to non-target DNA with 8 bp R-loop | Electron microscopy | 3.02 | 116 | 2024-05-01 |
18 | 8T6S|1|B+ 8T6S|1|C+ 8T6S|1|D | Trans-activating crRNA | gRNA, NTS, TS | Streptococcus pyogenes | Bacteria | RF02348 | SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 10 bp R-loop | Electron microscopy | 3.28 | 119 | 2024-05-01 |
19 | 8T77|1|B+ 8T77|1|C+ 8T77|1|D | Trans-activating crRNA | gRNA, NTS, TS | Streptococcus pyogenes | Bacteria | RF02348 | SpRY-Cas9:gRNA complex bound to non-target DNA with 6 bp R-loop | Electron microscopy | 3.25 | 114 | 2024-05-01 |
20 | 8T6Y|1|B | Trans-activating crRNA | gRNA | Streptococcus pyogenes | Bacteria | RF02348 | SpRY-Cas9:gRNA complex bound to non-target DNA with 1 bp R-loop | Electron microscopy | 2.88 | 88 | 2024-05-01 |
21 | 8T7S|1|H | Trans-activating crRNA | gRNA | Streptococcus pyogenes | Bacteria | RF02348 | SpRYmer bound to NAC PAM DNA | Electron microscopy | 3.01 | 88 | 2024-05-01 |
22 | 8T6P|1|B | Trans-activating crRNA | gRNA | Streptococcus pyogenes | Bacteria | RF02348 | SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 2 bp R-loop | Electron microscopy | 3.15 | 88 | 2024-05-01 |
23 | 8T76|1|B | Trans-activating crRNA | gRNA | Streptococcus pyogenes | Bacteria | RF02348 | SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 3 bp R-loop | Electron microscopy | 3.42 | 88 | 2024-05-01 |