HL_8KAL_001

3D structure

PDB id

8KAL (explore in PDB, NAKB, or RNA 3D Hub)

Description

Crystal structure of SpyCas9 in complex with sgRNA and 17nt target DNA

Experimental method

X-RAY DIFFRACTION

Resolution

3.16 Å

Loop

Sequence

UAGAAAUA

Length

8 nucleotides

Bulged bases

None detected

QA status

Valid loop

Sequence variability

If this chain is mapped to an Rfam alignment, the link below will give its sequence variability.

R3DSVS

Structural variability across Equivalence Class

The link below will give the loop's structural variability across the equivalence class for this chain.

R3DMCS EC

Structural variability across Rfam

If this chain is mapped to an Rfam alignment, the link below will give the loop's structural variability between chains mapped to the same Rfam family.

R3DMCS Rfam

Detailed Annotation

GNRA with extra cWW

Broad Annotation

GNRA with extra cWW

Motif group

HL_04259.3

Basepair signature

cWW-F-F-F-F-F-F

Number of instances in this motif group

7

Unit IDs

8KAL|1|A|U|31
8KAL|1|A|A|32
8KAL|1|A|G|33
8KAL|1|A|A|34
8KAL|1|A|A|35
8KAL|1|A|A|36
8KAL|1|A|U|37
8KAL|1|A|A|38

Current chains

Chain A

RNA (98-MER)

Nearby chains

Chain B

CRISPR-associated endonuclease Cas9/Csn1