IL_7QQP_008

3D structure

PDB id

7QQP (explore in PDB, NAKB, or RNA 3D Hub)

Description

SpCas9 bound to AAVS1 off-target3 DNA substrate

Experimental method

X-RAY DIFFRACTION

Resolution

2.6 Å

Loop

Sequence

ACA*TCT

Length

6 nucleotides

Bulged bases

None detected

QA status

Valid loop

Sequence variability

If this chain is mapped to an Rfam alignment, the link below will give its sequence variability.

R3DSVS

Structural variability across Equivalence Class

The link below will give the loop's structural variability across the equivalence class for this chain.

R3DMCS EC

Structural variability across Rfam

If this chain is mapped to an Rfam alignment, the link below will give the loop's structural variability between chains mapped to the same Rfam family.

R3DMCS Rfam

Detailed Annotation

Isolated non-canonical cWW pair

Broad Annotation

No text annotation

Motif group

IL_01003.3

Basepair signature

cWW-cWW-cWW

Number of instances in this motif group

214

Unit IDs

7QQP|1|A|A|14
7QQP|1|A|C|15
7QQP|1|A|A|16
*
7QQP|1|C|DT|5
7QQP|1|C|DC|6
7QQP|1|C|DT|7

Current chains

Chain A

AAVS1 sgRNA

Chain C

AAVS1 off-target3 target strand

Nearby chains

Chain B

CRISPR-associated endonuclease Cas9/Csn1