IL_7Z4D_016

3D structure

PDB id

7Z4D (explore in PDB, NAKB, or RNA 3D Hub)

Description

Crystal structure of SpCas9 bound to a 10 nucleotide complementary DNA substrate

Experimental method

X-RAY DIFFRACTION

Resolution

3.1 Å

Loop

Sequence

AAG*CGT

Length

6 nucleotides

Bulged bases

None detected

QA status

Valid loop

Sequence variability

If this chain is mapped to an Rfam alignment, the link below will give its sequence variability.

R3DSVS

Structural variability across Equivalence Class

The link below will give the loop's structural variability across the equivalence class for this chain.

R3DMCS EC

Structural variability across Rfam

If this chain is mapped to an Rfam alignment, the link below will give the loop's structural variability between chains mapped to the same Rfam family.

R3DMCS Rfam

Detailed Annotation

No text annotation

Broad Annotation

No text annotation

Motif group

IL_91129.1

Basepair signature

cWW-L-R-cWW

Number of instances in this motif group

10

Unit IDs

7Z4D|1|A|A|9
7Z4D|1|A|A|10
7Z4D|1|A|G|11
*
7Z4D|1|C|DC|10
7Z4D|1|C|DG|11
7Z4D|1|C|DT|12

Current chains

Chain A

sgRNA

Chain C

Target strand of 10 nucleotide complementary DNA substrate

Nearby chains

Chain B

CRISPR-associated endonuclease Cas9/Csn1

Chain D

Non-target strand of 10 nucleotide complementary DNA substrate