IL_8KAL_001

3D structure

PDB id

8KAL (explore in PDB, NAKB, or RNA 3D Hub)

Description

Crystal structure of SpyCas9 in complex with sgRNA and 17nt target DNA

Experimental method

X-RAY DIFFRACTION

Resolution

3.16 Å

Loop

Sequence

UUU*AAA

Length

6 nucleotides

Bulged bases

None detected

QA status

Self-complementary: UUU,AAA

Sequence variability

If this chain is mapped to an Rfam alignment, the link below will give its sequence variability.

R3DSVS

Structural variability across Equivalence Class

The link below will give the loop's structural variability across the equivalence class for this chain.

R3DMCS EC

Structural variability across Rfam

If this chain is mapped to an Rfam alignment, the link below will give the loop's structural variability between chains mapped to the same Rfam family.

R3DMCS Rfam

IL_8KAL_001 not in the Motif Atlas

Geometric match to IL_4LFB_047

Geometric discrepancy: 0.282

The information below is about IL_4LFB_047

Detailed Annotation

Isolated non-canonical cWW pair

Broad Annotation

No text annotation

Motif group

IL_68118.3

Basepair signature

cWW-cWW-cWW

Number of instances in this motif group

29

Unit IDs

8KAL|1|A|U|22
8KAL|1|A|U|23
8KAL|1|A|U|24
*
8KAL|1|A|A|47
8KAL|1|A|A|48
8KAL|1|A|A|49

Current chains

Chain A

RNA (98-MER)

Nearby chains

Chain B

CRISPR-associated endonuclease Cas9/Csn1

Chain C

DNA (25-MER)