IL_8KAL_007

3D structure

PDB id

8KAL (explore in PDB, NAKB, or RNA 3D Hub)

Description

Crystal structure of SpyCas9 in complex with sgRNA and 17nt target DNA

Experimental method

X-RAY DIFFRACTION

Resolution

3.16 Å

Loop

Sequence

GAAG*CTTC

Length

8 nucleotides

Bulged bases

None detected

QA status

Self-complementary:

Sequence variability

If this chain is mapped to an Rfam alignment, the link below will give its sequence variability.

R3DSVS

Structural variability across Equivalence Class

The link below will give the loop's structural variability across the equivalence class for this chain.

R3DMCS EC

Structural variability across Rfam

If this chain is mapped to an Rfam alignment, the link below will give the loop's structural variability between chains mapped to the same Rfam family.

R3DMCS Rfam

Detailed Annotation

No text annotation

Broad Annotation

No text annotation

Motif group

Not in a motif group

Basepair signature

Not available

Number of instances in this motif group

0

Unit IDs

8KAL|1|A|G|17
8KAL|1|A|A|18
8KAL|1|A|A|19
8KAL|1|A|G|20
*
8KAL|1|C|DC|9
8KAL|1|C|DT|10
8KAL|1|C|DT|11
8KAL|1|C|DC|12

Current chains

Chain A

RNA (98-MER)

Chain C

DNA (25-MER)

Nearby chains

Chain B

CRISPR-associated endonuclease Cas9/Csn1

Chain D

DNA (5'-D(*TP*TP*TP*AP*GP*GP*TP*AP*TP*TP*G)-3')