Structure title
Crystal structure of SpyCas9-crRNA-tracrRNA complex bound to 16nt target DNA
Authors
Chen, Y., Chen, J., Liu, L.
Release date
2024-06-05
Experimental technique
X-RAY DIFFRACTION
Resolution
3.4 Å
Chains
4 RNA chains from Streptococcus pyogenes serotype M1
4 DNA chains
2 other chains
Nucleic acid compounds
RNA (34-MER), RNA (34-MER), RNA (65-MER), RNA (65-MER), DNA (5'-D(*CP*AP*AP*TP*AP*CP*CP*TP*TP*TP*TP*AP*TP*CP*CP*AP*TP*AP*AP*AP*TP*TP*CP*G)-3'), DNA (5'-D(*TP*TP*TP*AP*GP*GP*TP*AP*TP*TP*G)-3'), DNA (5'-D(*CP*AP*AP*TP*AP*CP*CP*TP*TP*TP*TP*AP*TP*CP*CP*AP*TP*AP*AP*AP*TP*TP*CP*G)-3'), DNA (5'-D(*TP*TP*TP*AP*GP*GP*TP*AP*TP*TP*G)-3')
Other compounds
CRISPR-associated endonuclease Cas9/Csn1, CRISPR-associated endonuclease Cas9/Csn1

Pairwise Interactions

Interactions annotated by FR3D: View all

RNA 3D Motifs

In the current release of the RNA 3D Motif Atlas, 8KAJ contains:
  • 7 hairpin loops from 3 motif groups
  • 11 internal loops from 2 motif groups
  • 0 junction loops from 0 motif groups
More

3D Redundancy

8KAJ has chains which belong to the following equivalence classes in the current representative set:

Similar structures

None found
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