IL_8KAL_005

3D structure

PDB id

8KAL (explore in PDB, NAKB, or RNA 3D Hub)

Description

Crystal structure of SpyCas9 in complex with sgRNA and 17nt target DNA

Experimental method

X-RAY DIFFRACTION

Resolution

3.16 Å

Loop

Sequence

GC*GUC

Length

5 nucleotides

Bulged bases

8KAL|1|E|U|59

QA status

Valid loop

Sequence variability

If this chain is mapped to an Rfam alignment, the link below will give its sequence variability.

R3DSVS

Structural variability across Equivalence Class

The link below will give the loop's structural variability across the equivalence class for this chain.

R3DMCS EC

Structural variability across Rfam

If this chain is mapped to an Rfam alignment, the link below will give the loop's structural variability between chains mapped to the same Rfam family.

R3DMCS Rfam

IL_8KAL_005 not in the Motif Atlas

Geometric match to IL_5B2T_002

Geometric discrepancy: 0.1042

The information below is about IL_5B2T_002

Detailed Annotation

Single bulged U

Broad Annotation

No text annotation

Motif group

IL_97561.2

Basepair signature

cWW-L-cWW

Number of instances in this motif group

147

Unit IDs

8KAL|1|E|G|54
8KAL|1|E|C|55
*
8KAL|1|E|G|58
8KAL|1|E|U|59
8KAL|1|E|C|60

Current chains

Chain E

RNA (98-MER)

Nearby chains

Chain G

CRISPR-associated endonuclease Cas9/Csn1