5F9R Summary
- Structure title
- Crystal structure of catalytically-active Streptococcus pyogenes CRISPR-Cas9 in complex with single-guided RNA and double-stranded DNA primed for target DNA cleavage
- Authors
- Release date
- 2016-01-27
- Experimental technique
- X-RAY DIFFRACTION
- Resolution
- 3.4 Å
- Chains
- 1 RNA chain from Streptococcus pyogenes
-
2 DNA chains
- 1 other chain
- Nucleic acid compounds
- Other compounds
- RNA (116-MER), DNA (30-MER), DNA (5'-D(P*AP*TP*GP*AP*GP*AP*CP*GP*CP*TP*GP*GP*AP*GP*TP*AP*CP*AP*C)-3')
- CRISPR-associated endonuclease Cas9/Csn1
Pairwise Interactions
Interactions annotated by FR3D:- 56 base-pairs
- 164 base-stacking
- 1 base-phosphate
- 1 base-ribose
RNA 3D Motifs
In the current release of the RNA 3D Motif Atlas, 5F9R contains:- 4 hairpin loops from 3 motif groups
- 11 internal loops from 2 motif groups
- 0 junction loops from 0 motif groups
3D Redundancy
5F9R has chains which belong to the following equivalence classes in the current representative set:- NR_all_53718.2 (1) rep
- NR_4.0_53718.2 (1) rep
- NR_3.5_53718.2 (1) rep
- NR_20.0_53718.2 (1) rep