7S4U Summary
- Structure title
- Cryo-EM structure of Cas9 in complex with 12-14MM DNA substrate, 5 minute time-point
- Authors
- Bravo, J.P.K., Taylor, D.W., Liu, M.S., Johnson, K.A.
- Release date
- 2022-03-02
- Experimental technique
- ELECTRON MICROSCOPY
- Resolution
- 3.6 Å
- Chains
- 1 RNA chain from synthetic construct
-
2 DNA chains
- 1 other chain
- Nucleic acid compounds
- Other compounds
- gRNA, Target strand, Non-target strand
- CRISPR-associated endonuclease Cas9/Csn1
Pairwise Interactions
Interactions annotated by FR3D:- 41 base-pairs
- 134 base-stacking
- 2 base-phosphate
- 1 base-ribose
RNA 3D Motifs
In the current release of the RNA 3D Motif Atlas, 7S4U contains:- 4 hairpin loops from 0 motif groups
- 10 internal loops from 0 motif groups
- 0 junction loops from 0 motif groups
3D Redundancy
7S4U has chains which belong to the following equivalence classes in the current representative set:- NR_all_56990.5 (1)
- NR_4.0_56990.5 (1)
- NR_20.0_56990.5 (1)
Similar structures
5B2T 5B2R 5B2S 7Z4L 8SRS 6AI6 4OO8 7S4X 7Z4J 4OO8 9ED9 9EAL 9EAK 8YE6 8T6T 7Z4I 6O0X 6O0Y 6O0Z 8T6X 8T7S 8T6Y 9C9P 9EDA 9CGU 7S4V 8SPQ 9EDB 8T79 8T78 8U3Y 4ZT0 9JL2 8T6P 8T7S 4ZT0 4ZT9 7Z4D 8SQH 7Z4H 7Z4D 4ZT9 8T77 5XBL 8TZZ 8T6S 5F9R 7Z4C 7Z4G 8T76 6IFO 6MCB 7Z4K 9JL1 7Z4E 6MCC 6IFO 7V59 5VZLStructures from NR_all_56990.5